| Author's Name: |
Sonal S. Charde & Dr. Prakash R. Itankar |
| Subject Area: |
Health Science |
| Subject |
Pharmaceutical Science |
| Section |
Research Paper |
Keyword:
HPTLC, Rutin, Tephrosia Purpurea
Abstract
The present investigation was designed to evaluate the chromatographic separation of Rutin in hydroalcoholic extract and its different fractions such as chloroform (CHTP) , ethyl acetate (EATP), butanol soluble (BSTP), butanol insoluble fraction (BISTP). A simple, rapid HPTLC method had been developed for estimation of flavonoid rutin on aluminium baked plates coated with silica gel 60F254 as the stationary phase and mobile phase consist of ethyl acetate: glacial acetic acid: formic acid: water ( 100:11:11:27) for rutin . Detection and quantification were performed densitometrically at ?max 254 nm for rutin . The standard Rf value of rutin was found to be 0.52. The total peak areas of the standard rutin and the corresponding peak areas of extracts were compared and the highest rutin content was estimated to be 26.86 mg in butanol soluble (BSTP) fraction.