IMPACT OF BIOAGENTS AND ORGANIC ACIDS ON ALTERNARIA SOLANI, THE CAUSAL ORGANISM OF TOMATO EARLY BLIGHT DISEASE AND THEIR SIDE EFFECT ASSESSMENT

Author's Name: Moustafa M. S. H., Asmaa M. A. ALkolaly and Hala A. M. El-Dakar
Subject Area: Life Sciences
Subject Agricultural
Section Research Paper

Keyword:

Biocides, BioZied, BioArc, Salycalicacid, Humic acid, toxicological studies.


Abstract

Pesticides have a specific purpose in society; they are intended to control insects, fungi, bacteria and weeds that damage crops; however, pesticides can adversely affect some nontarget living organism. Even the least-toxic products, and those that are natural or organic products, can cause human health problems. In this work, we studied the effect of Bio Arc (Bacillus megaterium) a biocide, the resistance inducer “salicylic acid” and the growth promoter “humic acid” were studied on Alternaria solani the causal of tomato early blight disease, compared with the recommended biocide, Bio Zeid (Trichoderma album). Since the most of tomato fruits are permanently consumed fresh, therefore it was very important to assess the possible toxicological risk of long-term consumption of tomato fruits harvested from plants treated with any of the aforementioned biocides. In vivo study, Bio Arc was the most effective one (67.8%) followed by Bio Zeid (43%) then salicylic acid (40%), in contrast to humic acid, which resulted in very low efficacy (35.4%). However, while disease severity on plants treated with Bio Zeid increased gradually tell after the third spray, on plants treated with Bio Arc and salicylic acid increased gradually tell the second spray. feeding rats for 4 weeks on fodder, mixed with juice of tomato fruits harvested from plants treated with salicylic acid,Bio Zeid or Bio Arc compared with two control treatment (control+) and (control-) revealed that: in case of salicylic acid, no significant adverse effect was noticed on Alanineaminotransferase (ALT), High density lipoprotein (HDL) and albumin; in contrast to Aspertateaminotranferase (AST), cholesterol, triglyceride, Low density lipoprotein (LDL), creatinin and urea which were significantly increased .BioZeid showed no significant adverse effect on AST, cholesterol, HDL or albumin; in contrast to ALT, triglyceride, LDL, creatinin and urea .BioArc showed no significant adverse effect on ALT and AST, on the other hand, the levels of cholesterol, triglyceride, LDL, creatinin and urea significantly elevated. In contrast, HDL and albumin which significantly decreased. Elevation of AST and ALT in case of salicylic acid,Bio Zeid respectively indicated that, the rats liver may be hurt, especially liver weight of rats fed on fodder mixed with tomato treated with Bio Zied significantly decreased. Raised of creatinin and urea level in blood of rats fed on fodder mixed with tomato treated with salicylic acid ,BioZied or Bio Arc indicating that the rats kidneys may be adversely affected, especially kidney weight of rats fed on fodder mixed with tomato treated with Bio Zied significantly decreased.

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